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pnifty3 a seap  (InvivoGen)
90
InvivoGen pnifty3 a seap
MCF7 cells transfected with the <t>pNifty3-A-SEAP</t> reporter plasmid were pre-incubated or not (control) with 10 nM E2 for 24 h and stimulated with 100 ng/ml of flagellin for 24 h. Data of AP-1 activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).
Pnifty3 A Seap, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pnifty3 a seap/product/InvivoGen
Average 90 stars, based on 1 article reviews
pnifty3 a seap - by Bioz Stars, 2026-02
90/100 stars
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pnifty3  (InvivoGen)
90
InvivoGen pnifty3
MCF7 cells transfected with the <t>pNifty3-A-SEAP</t> reporter plasmid were pre-incubated or not (control) with 10 nM E2 for 24 h and stimulated with 100 ng/ml of flagellin for 24 h. Data of AP-1 activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).
Pnifty3, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pnifty3/product/InvivoGen
Average 90 stars, based on 1 article reviews
pnifty3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
pnifty3 i seap plasmid  (InvivoGen)
90
InvivoGen pnifty3 i seap plasmid
MCF7 cells transfected with the <t>pNifty3-A-SEAP</t> reporter plasmid were pre-incubated or not (control) with 10 nM E2 for 24 h and stimulated with 100 ng/ml of flagellin for 24 h. Data of AP-1 activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).
Pnifty3 I Seap Plasmid, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pnifty3 i seap plasmid/product/InvivoGen
Average 90 stars, based on 1 article reviews
pnifty3 i seap plasmid - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

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MCF7 cells transfected with the pNifty3-A-SEAP reporter plasmid were pre-incubated or not (control) with 10 nM E2 for 24 h and stimulated with 100 ng/ml of flagellin for 24 h. Data of AP-1 activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).

Journal: Scientific Reports

Article Title: Understanding the dynamics of Toll-like Receptor 5 response to flagellin and its regulation by estradiol

doi: 10.1038/srep40981

Figure Lengend Snippet: MCF7 cells transfected with the pNifty3-A-SEAP reporter plasmid were pre-incubated or not (control) with 10 nM E2 for 24 h and stimulated with 100 ng/ml of flagellin for 24 h. Data of AP-1 activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).

Article Snippet: TLR5 expression, NF-κB and AP-1 transcription activity were evaluated using MCF7 cells grown in 12-well plates and transiently transfected with hTLR5p-SEAP, pNifty2-SEAP or pNifty3-A-SEAP (Invivogen) reporter plasmids, respectively.

Techniques: Transfection, Plasmid Preparation, Incubation, Activity Assay

SEAP expression of MCF7 cells transfected with either the pNifty2-SEAP ( a and b ) or the pNifty3-A-SEAP ( c ) reporter plasmid. ( a and c ) Transfected MCF7 cells were pre-incubated with ER agonists PPT, DPN or G1 (0, 10, 100 and 1000 nM) for 24 h and then stimulated with 100 ng/ml of flagellin. ( b ) Effect of the ER blockade by specific ER antagonists (MPP, PHTPP or G15). A 2 × 2 factorial experimental design was used where transfected MCF7 cells were either: (1) cultured in the absence of both ER antagonist and E2 (control); (2) treated with 1 μM of ER antagonist for 30 min; (2) pre-incubated with 10 nM E2 for 24 h or (2) treated with 1 μM of ER antagonist for 30 min and then pre-incubated with 10 nM E2 for 24 h. The cells were then stimulated with 100 ng/ml of flagellin and analyzed at 24 h using NovaBrightTM Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection System 2.0. Control Data of NF-kB activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).

Journal: Scientific Reports

Article Title: Understanding the dynamics of Toll-like Receptor 5 response to flagellin and its regulation by estradiol

doi: 10.1038/srep40981

Figure Lengend Snippet: SEAP expression of MCF7 cells transfected with either the pNifty2-SEAP ( a and b ) or the pNifty3-A-SEAP ( c ) reporter plasmid. ( a and c ) Transfected MCF7 cells were pre-incubated with ER agonists PPT, DPN or G1 (0, 10, 100 and 1000 nM) for 24 h and then stimulated with 100 ng/ml of flagellin. ( b ) Effect of the ER blockade by specific ER antagonists (MPP, PHTPP or G15). A 2 × 2 factorial experimental design was used where transfected MCF7 cells were either: (1) cultured in the absence of both ER antagonist and E2 (control); (2) treated with 1 μM of ER antagonist for 30 min; (2) pre-incubated with 10 nM E2 for 24 h or (2) treated with 1 μM of ER antagonist for 30 min and then pre-incubated with 10 nM E2 for 24 h. The cells were then stimulated with 100 ng/ml of flagellin and analyzed at 24 h using NovaBrightTM Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection System 2.0. Control Data of NF-kB activity are reported as the fold induction of SEAP activity over untreated controls. Data are representative of at least five independent experiments. Error bars denote SEM. Different letters mean significant difference (p < 0.05).

Article Snippet: TLR5 expression, NF-κB and AP-1 transcription activity were evaluated using MCF7 cells grown in 12-well plates and transiently transfected with hTLR5p-SEAP, pNifty2-SEAP or pNifty3-A-SEAP (Invivogen) reporter plasmids, respectively.

Techniques: Expressing, Transfection, Plasmid Preparation, Incubation, Cell Culture, Activity Assay